Issue 39, 2015
From the journal:

Chemical Communications

Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines†

Rasa Rakauskaitė,a   Giedrė Urbanavičiūtė,a   Audronė Rukšėnaitė,a   Zita Liutkevičiūtė,‡a   Robertas Juškėnas,ab   Viktoras Masevičiusab  and  Saulius Klimašauskas*a  

* Corresponding authors

a Institute of Biotechnology, Vilnius University, V. Graičiūno 8, Vilnius LT-02241, Lithuania
E-mail: saulius.klimasauskas@bti.vu.lt
Fax: +370 5260116

b Faculty of Chemistry, Vilnius University, Naugarduko 24, Vilnius LT-03225, Lithuania

Abstract

Selenocysteine is a valuable component of both natural selenoproteins and designer biocatalysts; however the availability of such proteins is hampered by technical limitations. Here we report the first general strategy for the production of selenoproteins via genetically-encoded incorporation of a synthetic photocaged selenocysteine residue in yeast cells, and provide examples of light-controlled protein dimerization and targeted covalent labeling in vitro.

Graphical abstract: Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C4CC07910H
Article type
Communication
Submitted
07 Nhl 2014
Accepted
05 Yan 2015
First published
05 Yan 2015
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2015,51, 8245-8248

Permissions

Request permissions

Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines

R. Rakauskaitė, G. Urbanavičiūtė, A. Rukšėnaitė, Z. Liutkevičiūtė, R. Juškėnas, V. Masevičius and S. Klimašauskas, Chem. Commun., 2015, 51, 8245 DOI: 10.1039/C4CC07910H

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements