Issue 12, 2024

Collagen-decorated electrospun scaffolds of unsaturated copolyesters for bone tissue regeneration

Abstract

Many efforts have been devoted to bone tissue to regenerate damaged tissues, and the development of new biocompatible materials that match the biological, mechanical, and chemical features required for this application is crucial. Herein, a collagen-decorated scaffold was prepared via electrospinning using a synthesized unsaturated copolyester (poly(globalide-co-pentadecalactone)), followed by two coupling reactions: thiol–ene functionalization with cysteine and further conjugation via EDC/NHS chemistry with collagen, aiming to design a bone tissue regeneration device with improved hydrophilicity and cell viability. Comonomer ratios were varied, affecting the copolymer's thermal and chemical properties and highlighting the tunable features of this copolyester. Functionalization with cysteine created new carboxyl and amine groups needed for bioconjugation with collagen, which is responsible for providing biological and structural integrity to the extra-cellular matrix. Bioconjugation with collagen turned the scaffold highly hydrophilic, decreasing its contact angle from 107 ± 2° to 0°, decreasing the copolymer crystallinity by 71%, and improving cell viability by 85% compared with the raw scaffold, thus promoting cell growth and proliferation. The highly efficient and biosafe strategy to conjugate polymers and proteins created a promising device for bone repair in tissue engineering.

Graphical abstract: Collagen-decorated electrospun scaffolds of unsaturated copolyesters for bone tissue regeneration

Supplementary files

Article information

Article type
Paper
Submitted
09 Huk 2023
Accepted
17 Yan 2024
First published
20 Yan 2024

J. Mater. Chem. B, 2024,12, 3047-3062

Collagen-decorated electrospun scaffolds of unsaturated copolyesters for bone tissue regeneration

H. B. Madalosso, C. Guindani, B. C. Maniglia, P. H. Hermes de Araújo and C. Sayer, J. Mater. Chem. B, 2024, 12, 3047 DOI: 10.1039/D3TB02665E

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