Issue 34, 2022

Preparation of tannic acid and l-cysteine functionalized magnetic composites for synergistic enrichment of N-glycopeptides followed by mass spectrometric analysis

Abstract

Glycoprotein is involved in a variety of biological activities and has been linked to a number of diseases. Glycopeptide enrichment prior to mass spectrometry (MS) detection is crucial to reduce interference, improve detection efficiency, and analyze proteomics deeply and comprehensively. Here, we prepared a novel magnetic hydrophilic material combining tannic acid (TA) and L-cysteine (L-Cys) through a simple and fast procedure. Owing to the synergistic hydrophilic interaction of TA and L-Cys, the obtained adsorbent material shows excellent enrichment performance toward N-glycopeptides with low detection limit, high selectivity, and good reusability. Besides, the material can also be utilized for the enrichment of N-glycopeptides in human serum and saliva, which shows its application prospect in complex biological samples.

Graphical abstract: Preparation of tannic acid and l-cysteine functionalized magnetic composites for synergistic enrichment of N-glycopeptides followed by mass spectrometric analysis

Supplementary files

Article information

Article type
Paper
Submitted
21 Maw 2022
Accepted
05 Mha 2022
First published
15 Mha 2022

Anal. Methods, 2022,14, 3260-3269

Preparation of tannic acid and L-cysteine functionalized magnetic composites for synergistic enrichment of N-glycopeptides followed by mass spectrometric analysis

Y. Wang, W. Xu, H. Xu and Q. Jia, Anal. Methods, 2022, 14, 3260 DOI: 10.1039/D2AY01169G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements