Issue 3, 2018

Graphene oxide-mediated Cas9/sgRNA delivery for efficient genome editing

Abstract

Direct cellular delivery of CRISPR/Cas9 complexes is of great significance for genome editing and other recently developed applications, such as gene expression regulation and RNA/DNA imaging. Here, we first constructed a graphene oxide (GO)-polyethylene glycol (PEG)-polyethylenimine (PEI) nanocarrier for the delivery of high-molecular-weight Cas9/single-guide RNA (sgRNA) complexes for endocytosis, endosomal escape, nuclear entry, and gene editing. The results demonstrate that the nanocarrier can be used successfully for efficient gene editing in human AGS cells with an efficiency of ∼39%. The results also show that this nanocarrier can protect sgRNA from enzymatic degradation, thus exhibiting extremely high stability, which is critical for future in vivo applications. Thus, this GO-mediated Cas9/sgRNA delivery system has potential as a new approach for biomedical research and targeted gene engineering applications.

Graphical abstract: Graphene oxide-mediated Cas9/sgRNA delivery for efficient genome editing

Supplementary files

Article information

Article type
Paper
Submitted
27 Nhl 2017
Accepted
17 N’w 2017
First published
18 N’w 2017

Nanoscale, 2018,10, 1063-1071

Graphene oxide-mediated Cas9/sgRNA delivery for efficient genome editing

H. Yue, X. Zhou, M. Cheng and D. Xing, Nanoscale, 2018, 10, 1063 DOI: 10.1039/C7NR07999K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements