Issue 94, 2017

Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase

Abstract

The possibility to sequence cytotoxic O6-alkylG DNA adducts would greatly benefit research. Recently we reported a benzimidazole-derived nucleotide that is selectively incorporated opposite the damaged site by a mutated DNA polymerase. Here we provide the structural basis for this reaction which may spur future developments in DNA damage sequencing.

Graphical abstract: Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase

Supplementary files

Article information

Article type
Communication
Submitted
13 Ndz 2017
Accepted
02 Huk 2017
First published
07 Huk 2017

Chem. Commun., 2017,53, 12704-12707

Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase

K. Betz, A. Nilforoushan, L. A. Wyss, K. Diederichs, S. J. Sturla and A. Marx, Chem. Commun., 2017, 53, 12704 DOI: 10.1039/C7CC07173F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements