Issue 29, 2021

Dansyl–NA3 conjugates for glycoprotein detection through fluorescent tagging and native gel electrophoresis

Abstract

A new approach to the fluorescent labelling of glycoproteins was developed utilizing the aldehyde-reactive N-amino anthranilic acid (NA3) moiety linked to a dansyl fluorophore. The presented synthetic method is based on the conjugation of isatin as a “masked” anthranilic acid, thus eliminating the need for the use of protecting groups. Fluorescence properties and chemical stability of the prepared molecules were evaluated, resulting in the selection of a conjugate directly connecting the dansyl moiety and aldehyde-reactive NA3 through a sulfonamide linkage for further studies. NaIO4-mediated oxidation of the glycosylated protein fetuin followed by rapid fluorescent tagging and native gel electrophoresis afforded glycoprotein detection. The detection limit of the described method was 18 ng of protein, providing sensitivity sufficient for the detection of abundant glycosylated serum proteins.

Graphical abstract: Dansyl–NA3 conjugates for glycoprotein detection through fluorescent tagging and native gel electrophoresis

Supplementary files

Article information

Article type
Paper
Submitted
15 May 2021
Accepted
01 Tem 2021
First published
01 Tem 2021

New J. Chem., 2021,45, 13185-13195

Dansyl–NA3 conjugates for glycoprotein detection through fluorescent tagging and native gel electrophoresis

M. Suchý, A. Kirby, T. Sabloff, E. E. Mulvihill and A. J. Shuhendler, New J. Chem., 2021, 45, 13185 DOI: 10.1039/D1NJ02393D

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