Issue 43, 2017

pH-Sensing fluorescence oligonucleotide probes based on an i-motif scaffold: a review

Abstract

Fluorescence-based approaches have found widespread applications in cell biology. pH-Sensing using fluorescent i-motifs is a potentially useful strategy for real-time, in vivo monitoring of important cellular processes. i-Motif scaffolds can serve as the recognition element for H+ and are highly programmable, which leads to probes that exhibit different dynamic pH ranges and possess slightly different transition midpoints. Moreover, various methodologies can be employed to obtain fluorescence signals, such as incorporation of fluorescent cytosine analogues and excimers or FRET labeling. An alternative label-free strategy for i-motif formation and as a consequence, for pH monitoring, is also presented within this review. Strategies used for the internalization of i-motif sensors are cited as well as an example of anchoring them to a cell membrane for extracellular measurements is described. In this review, we have focused on recent developments in pH-sensing fluorescence oligonucleotide probes based on an i-motif scaffold, especially in the context of intracellular sensing and bioimaging.

Graphical abstract: pH-Sensing fluorescence oligonucleotide probes based on an i-motif scaffold: a review

Article information

Article type
Critical Review
Submitted
09 Ağu 2017
Accepted
28 Eyl 2017
First published
28 Eyl 2017

Anal. Methods, 2017,9, 6092-6106

pH-Sensing fluorescence oligonucleotide probes based on an i-motif scaffold: a review

A. Dembska, P. Bielecka and B. Juskowiak, Anal. Methods, 2017, 9, 6092 DOI: 10.1039/C7AY01942D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements