Issue 11, 2023

Antimicrobial peptide-functionalized polyhydroxyalkanoate bio-beads as a bactericidal material for water disinfection

Abstract

Waterborne diseases are a major public health concern worldwide. Water disinfection is being revolutionized by a new generation of technologies which aim to be effective in inactivating pathogens with minimum chemical and energy input and low risk to the ecosystem and human health. In this study, we created a new bactericidal material by expressing and displaying an antimicrobial peptide HHC10 on the surface of polyhydroxyalkanoate (PHA) bio-beads using synthetic biology. The material, named as bio-beads displayed HHC10 (BDH), had antimicrobial activity against Escherichia coli and Bacillus subtilis with reduction efficiencies of up to 6 log and 4 log, respectively. BDH retained 88% of the initial antimicrobial activity after 35 days storage, while the activity of free HHC10 peptide declined to 61%. BDH was effective in removing E. coli and B. subtilis in real-world surface water samples and had higher bacterial reduction efficiency than that in PBS buffer. Experimental results provided evidence that ionic strength is a key factor affecting the antimicrobial activity of BDH. This study reports, for the first time, the development of antimicrobial peptide-functionalized bio-beads as an effective bactericidal material, which can contribute to the development of future biomaterial-based technology for water disinfection.

Graphical abstract: Antimicrobial peptide-functionalized polyhydroxyalkanoate bio-beads as a bactericidal material for water disinfection

Supplementary files

Article information

Article type
Paper
Submitted
29 พ.ค. 2566
Accepted
26 ก.ย. 2566
First published
27 ก.ย. 2566

Environ. Sci.: Water Res. Technol., 2023,9, 2880-2890

Antimicrobial peptide-functionalized polyhydroxyalkanoate bio-beads as a bactericidal material for water disinfection

Y. Wu, B. Zhu and N. Wei, Environ. Sci.: Water Res. Technol., 2023, 9, 2880 DOI: 10.1039/D3EW00396E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements