Volume 222, 2020

Dual-emission fluorescent silicon nanoparticle-based nanothermometer for ratiometric detection of intracellular temperature in living cells

Abstract

In this article, we present a kind of dual-emission fluorescent nanothermometer, which is made of europium (Eu3+)-doped silicon nanoparticles (Eu@SiNPs), allowing the detection of intracellular temperature in living cells with high accuracy. In particular, the presented SiNP-based thermometer features dual-emission fluorescence (blue (455 nm) and red (620 nm) emission), negligible toxicity (cell viability of treated cells remains above 90% during 24 h of treatment) and robust photostability in living cells (i.e., preserving >90% of fluorescence intensity after 45 min of continuous UV irradiation). More significantly, the fluorescence intensity of the Eu@SiNPs exhibits a linear ratiometric temperature response in a broad range from 25 to 70 °C. Taking advantage of these attractive merits, the Eu@SiNP-based nanothermometer is able to accurately (∼4.5% change per °C) determine dynamic changes in intracellular temperature in a quantitative and long-term (i.e., 30 min) manner.

Graphical abstract: Dual-emission fluorescent silicon nanoparticle-based nanothermometer for ratiometric detection of intracellular temperature in living cells

Associated articles

Supplementary files

Article information

Article type
Paper
Submitted
20 ก.ย. 2562
Accepted
24 ต.ค. 2562
First published
25 ต.ค. 2562

Faraday Discuss., 2020,222, 122-134

Dual-emission fluorescent silicon nanoparticle-based nanothermometer for ratiometric detection of intracellular temperature in living cells

J. Wang, A. Jiang, J. Wang, B. Song and Y. He, Faraday Discuss., 2020, 222, 122 DOI: 10.1039/C9FD00088G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements