Issue 53, 2017

Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

Abstract

We expand photochromic super-resolution optical fluctuation imaging (pcSOFI) to monochromatic dual-channel sub-diffraction microscopy. Multi-tau (mt-)pcSOFI unmixes spectrally identical reversibly switchable fluorescent proteins (RSFPs) based on their blinking kinetics. We show that mt-pcSOFI can be used to simultaneously image two structures in living cells with existing RSFPs and the newly developed ffDronpa-F.

Graphical abstract: Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

Supplementary files

Article information

Article type
Communication
Submitted
27 มี.ค. 2560
Accepted
19 พ.ค. 2560
First published
31 พ.ค. 2560

Chem. Commun., 2017,53, 7242-7245

Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

S. Duwé, W. Vandenberg and P. Dedecker, Chem. Commun., 2017, 53, 7242 DOI: 10.1039/C7CC02344H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements