Light-induced stabilization of microtubules by photo-crosslinking of a Tau-derived peptide

Soei Watari; Hiroshi Inaba; Tomonori Tamura; Arif Md. Rashedul Kabir; Akira Kakugo; Kazuki Sada; Itaru Hamachi; Kazunori Matsuura

doi:10.1039/D2CC01890J

Light-induced stabilization of microtubules by photo-crosslinking of a Tau-derived peptide†

Soei Watari,^a Hiroshi Inaba,

*^ab Tomonori Tamura,

^c Arif Md. Rashedul Kabir,

^d Akira Kakugo,

^de Kazuki Sada,^de Itaru Hamachi

^cf and Kazunori Matsuura

*^ab

Author affiliations

* Corresponding authors

^a Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan
E-mail: hinaba@tottori-u.ac.jp, ma2ra-k@tottori-u.ac.jp

^b Centre for Research on Green Sustainable Chemistry, Tottori University, Tottori 680-8552, Japan

^c Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan

^d Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan

^e Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo 060-0810, Japan

^f JST-ERATO, Hamachi Innovative Molecular Technology for Neuroscience, Nishikyo-ku, Kyoto 615-8530, Japan

Abstract

For light-induced stabilization of microtubules (MTs) to manipulate cells, a photo-reactive diazirine group was conjugated to a Tau-derived peptide, a motif binding on the inside of MTs. Ultraviolet (UV) light irradiation induced significant stabilization of MTs via the formation of a covalent bond of the peptide and showed toxicity.

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DOI: https://doi.org/10.1039/D2CC01890J
Article type: Communication
Submitted: 02 ஏப்ரல் 2022
Accepted: 25 ஜூலை 2022
First published: 05 ஆகஸ்ட் 2022
This article is Open Access

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Chem. Commun., 2022,58, 9190-9193

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Light-induced stabilization of microtubules by photo-crosslinking of a Tau-derived peptide

S. Watari, H. Inaba, T. Tamura, A. Md. R. Kabir, A. Kakugo, K. Sada, I. Hamachi and K. Matsuura, Chem. Commun., 2022, 58, 9190 DOI: 10.1039/D2CC01890J

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Chemical Communications

Light-induced stabilization of microtubules by photo-crosslinking of a Tau-derived peptide†

Abstract

Supplementary files

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Light-induced stabilization of microtubules by photo-crosslinking of a Tau-derived peptide

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