Tutorial Review
Exploring the diagnostic synergy of isothermal amplification-integrated CRISPR technology for tuberculosis: a systematic review
To address the problems linked with Mycobacterium tuberculosis (MTB) detection, we need an accurate, sensitive, and rapid detection method for efficient epidemiological management of tuberculosis (TB).
Tutorial Review
Current advance of CRISPR/Cas-based SERS technology
This review summarizes the applications of CRISPR/Cas technology combined with SERS for nucleic acid detection, the operation of CRISPR/Cas technology, elements of SERS sensors, and provides an outlook on future developments.
Critical Review
Microfluidic platforms for CRISPR-based biosensing advancing molecular diagnostics from benchtop to point-of-care
This review highlights recent advances in microfluidics-integrated CRISPR–Cas systems for rapid, sensitive, and portable detection of diverse biomarkers, emphasizing their potential to enable sample-to-answer point-of-care diagnostics.
Critical Review
CRISPR-based diagnostics for circulating cell-free DNA: a paradigm shift in precision oncology
CRISPR-Cas technology is being integrated into advanced biosensing platforms, creating powerful tools for the rapid, precise, and sensitive cfDNA-based cancer detection.
Communication
A CRISPR-amplified label-free electrochemical aptasensor for the sensitive detection of HbA1c
A CRISPR-amplified electrochemical aptasensor was designed for highly sensitive and selective detection of HbA1c in ideal buffer and real samples.
Paper
CRISPR/Cas13a-assisted amplification-free miRNA biosensor via dark-field imaging and magnetic gold nanoparticles
CRISPR/Cas13a-based rapid amplification-free miRNA detection using a portable dark-field imaging system in 30 min.
About this collection
This themed collection explores the multifaceted relationship between the CRISPR/Cas system and molecular diagnostics, addressing current challenges and knowledge gaps in this field.
This collection is guest edited by Prof. Long Ma (https://orcid.org/0000-0001-8479-2663), Prof. Xiaoming Zhou (https://orcid.org/0000-0001-5597-4804) and Dr. Xinkuan Cheng (https://orcid.org/0000-0002-6382-9735) highlights the complex interactions between CRISPR-based diagnostics and healthcare. The aim of this collection was to provide valuable insights and practical significance to promote the development of CRISPR-based diagnostics within the field.