Engineering of halohydrin dehalogenases for the regio- and stereoselective synthesis of (S)-4-aryl-2-oxazolidinones

Jinsong Song, Chuanhua Zhou, Xi Chen, Yang Gu, Feng Xue, Qiaqing Wu and Dunming Zhu

Catal. Sci. Technol., 2024, 14, 1967
DOI: 10.1039/D3CY01584J

Chemo-catalytic synthesis of biomass-derived furanyl diethers: green and renewable bio-diesel components

Yujia Pang, Zhenlei Zhang, Weiwei Qiu, Ning Chen, Junnan Wei, Xiujuan Li, Lu Lin and He Huang

Sustainable Energy Fuels, 2022, 6, 4845
DOI: 10.1039/D2SE01026G

Computational design of an imine reductase: mechanism-guided stereoselectivity reversion and interface stabilization

Kai Wu, Jinrong Yan, Qinde Liu, Xiaojing Wang, Piaoru Wu, Yiyang Cao, Xiuhong Lu, Yixin Xu, Junhai Huang and Lei Shao

Chem. Sci., 2024, 15, 1431
DOI: 10.1039/D3SC04636B

Ferulic acid production in Escherichia coli by engineering caffeic acid O-methyltransferase

Di Qiu, Haifeng Ding, Chao Zhou, Zhenling Hou and Genlin Zhang

International Journal of Biological Macromolecules, 2025, 306, 141764
DOI: 10.1016/j.ijbiomac.2025.141764

Reshaping the active pocket of an aldo-keto reductase to enhance its detoxification activity towards 3-keto-deoxynivalenol

Jiafeng Niu, Bin Ma, Hao Zhu, Huawei Liu, Haizhen Zhao, Xiaomei Bie, Fengxia Lu and Ping Zhu

Food Science and Human Wellness, 2025, 14, 9250194
DOI: 10.26599/FSHW.2024.9250194

Rational Engineering of Self-Sufficient P450s to Boost Catalytic Efficiency of Carbene-Mediated C–S Bond Formation

Binhao Wang, Tai-Ping Zhou, Yu Shen, Jie Hu, Jieyu Zhou, Jin Tang, Ruizhi Han, Guochao Xu, Ulrich Schwaneberg, Binju Wang and Ye Ni

ACS Catal., 2025, 15, 5993
DOI: 10.1021/acscatal.5c01569

Stereochemistry in Asymmetric Reduction of Bulky–Bulky Ketones by Alcohol Dehydrogenases

Jieyu Zhou, Guochao Xu and Ye Ni

ACS Catal., 2020, 10, 10954
DOI: 10.1021/acscatal.0c02646

Enantioselective transformation of phytoplankton-derived dihydroxypropanesulfonate by marine bacteria

Le Liu, Xiang Gao, Changjie Dong, Huanyu Wang, Xiaofeng Chen, Xiaoyi Ma, Shujing Liu, Quanrui Chen, Dan Lin, Nianzhi Jiao and Kai Tang

The ISME Journal, 2024, 18
DOI: 10.1093/ismejo/wrae084

Surfactant-tolerance evolution of Bacillus clausii protease for enhancing activity and stability by reshaping the substrate access tunnel

Xiangyang Ma, Liya Wang, Jingyu Chen, Enping Guo, Hongchen Zheng, Lei Zhao, Fuping Lu and Yihan Liu

Colloids and Surfaces B: Biointerfaces, 2025, 245, 114289
DOI: 10.1016/j.colsurfb.2024.114289

Structural Analysis of Marine Flavobacterium Protein Glutaminase Reveals a “Gatekeeper” Residue Affecting Its Catalytic Activity

Yating Long, Shudong Peng, Ying Zhou, Huifang Zhang, Ge Zhao and Yonghua Wang

J. Agric. Food Chem., 2024, 72, 27504
DOI: 10.1021/acs.jafc.4c04471

Studies on Insertion/Deletion Residues for Functional Analysis and Improved Amination Activity in Meso-DAPDH from Corynebacterium glutamicum

Yaning Zhang, Jiaying Hao, Yongjun Cao, Wenjun Zhao, Hankun Liu, Xiuzhen Gao and Qinyuan Ma

Catalysts, 2024, 14, 220
DOI: 10.3390/catal14040220

Modifying Corynebacterium glutamicum by metabolic engineering for efficient synthesis of l-lysine

Zhenyang Liu, Jie Liu, Feng Zhang and Weiguo Zhang

Syst Microbiol and Biomanuf, 2025, 5, 288
DOI: 10.1007/s43393-024-00235-1

Highly enantioselective reduction of benzophenones by engineered Geotrichum candidum alcohol dehydrogenase

Zhongyao Tang, Guillermo Germán Otárola Tejada, Afifa Ayu Koesoema and Tomoko Matsuda

Appl Microbiol Biotechnol, 2026, 110
DOI: 10.1007/s00253-026-13717-0

Substrate-Cofactor Synergistic Reorientation and Substrate Tunnel Engineering of SspLCD for Efficient l-Pipecolic Acid Biosynthesis

Weijie Gao, Ziyan Huang, Yanjun Li, Shuya Sun, Qinghai Liu, Hualei Wang, Rong Chen and Dongzhi Wei

Org. Lett., 2025, 27, 8470
DOI: 10.1021/acs.orglett.5c02297

Classification and functional origins of stereocomplementary alcohol dehydrogenases for asymmetric synthesis of chiral secondary alcohols: A review

Lu Zhang, Zewen Sun, Guochao Xu and Ye Ni

International Journal of Biological Macromolecules, 2024, 270, 132238
DOI: 10.1016/j.ijbiomac.2024.132238

Improving the Product Specificity of Maltotetraose-Forming Amylase from Pseudomonas saccharophila STB07 by Removing the Carbohydrate-Binding Module

Kaiwen Duan, Xiaofeng Ban, Yinglan Wang, Caiming Li, Zhengbiao Gu and Zhaofeng Li

J. Agric. Food Chem., 2022, 70, 13709
DOI: 10.1021/acs.jafc.2c05580

Single‐Point Mutant Inverts the Stereoselectivity of a Carbonyl Reductase toward β‐Ketoesters with Enhanced Activity

Aipeng Li, Ting Wang, Qing Tian, Xiaohong Yang, Dongming Yin, Yong Qin and Lianbing Zhang

Chemistry A European J, 2021, 27, 6283
DOI: 10.1002/chem.202005195

Benzaldehyde derivatives on tin electroplating as corrosion resistance for fabricating copper circuit

Yunzhong Huang, Chao Yang, Xiang Tan, Zhenhai Zhang, Shouxu Wang, Jiacong Hu, Wei He, Zhuoming Du, Yongjie Du, Yao Tang, Xinhong Su and Yuanming Chen

Nanotechnology Reviews, 2022, 11, 3125
DOI: 10.1515/ntrev-2022-0497

Biocatalytic preparation of a key intermediate of antifungal drugs using an alcohol dehydrogenase with high organic tolerance

Jinrong Yan, Xiaojing Wang, Fangling Li, Lei Yang, Guixiang Shi, Weihang Sun, Lei Shao, Junhai Huang and Kai Wu

Tetrahedron Letters, 2021, 84, 153455
DOI: 10.1016/j.tetlet.2021.153455

Substrate expansion of Geotrichum candidum alcohol dehydrogenase towards diaryl ketones by mutation

Zhongyao Tang, Yuuki Takagi, Afifa Ayu Koesoema and Tomoko Matsuda

Appl Microbiol Biotechnol, 2024, 108
DOI: 10.1007/s00253-024-13375-0

Two anti‐Prelog NAD‐Dependent Alcohol Dehydrogenases with Broad Substrate Scope and Excellent Enantioselectivity

Matteo Damian and Francesco G. Mutti

Eur J Org Chem, 2023, 26
DOI: 10.1002/ejoc.202300734

Engineering substrate specificity of quinone-dependent dehydrogenases for efficient oxidation of deoxynivalenol to 3-keto-deoxynivalenol

Bin Ma, Jiafeng Niu, Hao Zhu, Huibing Chi, Zhaoxin Lu, Fengxia Lu and Ping Zhu

International Journal of Biological Macromolecules, 2024, 264, 130484
DOI: 10.1016/j.ijbiomac.2024.130484

Efficient Biocatalytic Synthesis of (R)-2-Chloro-1-(3,4-difluorophenyl)ethanol by the Short-Chain Dehydrogenase PpKR8 from Paraburkholderia phymatum STM815

Ling He, Wenjie Ye, Youyu Xie, Qinghai Liu, Hualei Wang and Dongzhi Wei

Org. Process Res. Dev., 2022, 26, 278
DOI: 10.1021/acs.oprd.1c00189

Engineering an alcohol dehydrogenase from Gluconobacter oxydans for improved production of a bulky Ezetimibe intermediate

Yuqinxin Xie, Dongzhi Wei and Jinping Lin

Molecular Catalysis, 2024, 569, 114586
DOI: 10.1016/j.mcat.2024.114586

The hidden hand of molecular chirality in marine biogeochemistry

Le Liu, Maosheng Zhong, Quanrui Chen and Kai Tang

Trends in Chemistry, 2025, 7, 149
DOI: 10.1016/j.trechm.2025.01.007

Single-point mutation to realize the switch between “specificity” and “promiscuity” in glucose dehydrogenase

Yunye wang, Mengting Li, Yinan Li, Jiuming Zhang, Di Wang, Qingyu Wang, Jingping Ouyang and Song You

Molecular Catalysis, 2025, 583, 115233
DOI: 10.1016/j.mcat.2025.115233

Biocatalytic characterization of an alcohol dehydrogenase variant deduced from Lactobacillus kefir in asymmetric hydrogen transfer

Aleksandra Rudzka, Beata Zdun, Natalia Antos, Lia Martínez Montero, Tamara Reiter, Wolfgang Kroutil and Paweł Borowiecki

Commun Chem, 2023, 6
DOI: 10.1038/s42004-023-01013-1

Acetoacetyl-CoA reductase PhaB as an excellent anti-Prelog biocatalyst for the synthesis of chiral β-hydroxyl ester and the molecular basis of its catalytic performance

Ting Wang, Ke Yang, Qing Tian, Ruiting Han, Xuanshuo Zhang, Aipeng Li and Lianbing Zhang

Molecular Catalysis, 2021, 514, 111854
DOI: 10.1016/j.mcat.2021.111854

From discovery to application: Enabling technology-based optimizing carbonyl reductases biocatalysis for active pharmaceutical ingredient synthesis

Jie Gu, Wanmeng Mu, Yan Xu and Yao Nie

Biotechnology Advances, 2025, 79, 108496
DOI: 10.1016/j.biotechadv.2024.108496

Enhancing Alcohol Dehydrogenase Activity for the Efficient Synthesis of (S)-2-Chloro-1-(2,4-dichlorophenyl)ethanol Using Computer-Aided Combinational Mutagenesis

Wenjie Ye, Jingwen Xie, Weijie Gao, Yifang Meng, Qinghai Liu, Hualei Wang and Dongzhi Wei

J. Agric. Food Chem., 2025, 73, 22554
DOI: 10.1021/acs.jafc.5c00174

Comprehensive screening strategy coupled with structure-guided engineering of l-threonine aldolase from Pseudomonas putida for enhanced catalytic efficiency towards l-threo-4-methylsulfonylphenylserine

Lihong Li, Rongzhen Zhang, Yan Xu and Wenchi Zhang

Front. Bioeng. Biotechnol., 2023, 11
DOI: 10.3389/fbioe.2023.1117890

Engineering a Carbonyl Reductase as a Potential Tool for the Synthesis of Chiral α‐Tetralinols

Aipeng Li, Wang Ting, Ke Yang, Xuanshuo Zhang, Dongming Yin, Yong Qin and Lianbing Zhang

ChemCatChem, 2021, 13, 4625
DOI: 10.1002/cctc.202100890

Computational Redesign of the Substrate Binding Pocket of Glutamate Dehydrogenase for Efficient Synthesis of Noncanonical l-Amino Acids

Ziyuan Wang, Haisheng Zhou, Haoran Yu, Zhongji Pu, Jinling Xu, Hongyu Zhang, Jianping Wu and Lirong Yang

ACS Catal., 2022, 12, 13619
DOI: 10.1021/acscatal.2c04636

Chemoenzymatic approach towards the synthesis of the antitumor and antileishmanial marine metabolite (+)-Harzialactone A via the stereoselective, biocatalyzed reduction of a prochiral ketone

Angelica Artasensi, Ivan Bassanini, E. E. Ferrandi, Lucia Feni, Giulio Vistoli, Laura Fumagalli and Raffaella Gandolfi

Bioorganic Chemistry, 2023, 138, 106675
DOI: 10.1016/j.bioorg.2023.106675

Biocatalytic asymmetric reduction of prochiral bulky-bulky ketones

Auwal Eshi Sardauna, Muhammad Abdulrasheed, Alexis Nzila and Musa M. Musa

Molecular Catalysis, 2023, 541, 113099
DOI: 10.1016/j.mcat.2023.113099

Resin-Immobilized Palladium Acetate and Alcohol Dehydrogenase for Chemoenzymatic Enantioselective Synthesis of Chiral Diarylmethanols

Yanyan Li, Guanhua Liu, Liya Zhou, Li Ma, Ying He, Jing Gao, Yanjun Jiang, Limei Ren and Yunting Liu

J. Org. Chem., 2024, 89, 4818
DOI: 10.1021/acs.joc.4c00023

The critical role of residues Phe120 and Val161 of (2 R,3 R)‑2,3‑butanediol dehydrogenase from Neisseria gonorrhoeae as probed by molecular docking and site‐directed mutagenesis

Xue Dong, Tingting Zhang, Chuanyue Gui, Shuping Fei, Haonan Xu, Jianrong Chang, Chaoqun Lian and Wanggang Tang

J Basic Microbiol, 2024, 64
DOI: 10.1002/jobm.202300751

Improving the Cβ Stereoselectivity of l‐Threonine Aldolase for the Synthesis of l‐threo‐4‐Methylsulfonylphenylserine by Modulating the Substrate‐Binding Pocket To Control the Orientation of the Substrate Entrance

Lichao Wang, Lian Xu, Bingmei Su, Wei Lin, Xinqi Xu and Juan Lin

Chemistry A European J, 2021, 27, 9654
DOI: 10.1002/chem.202100752

Reshaping Substrate-Binding Pocket of Leucine Dehydrogenase for Bidirectionally Accessing Structurally Diverse Substrates

Tao Wu, Yinmiao Wang, Ningxin Zhang, Dejing Yin, Yan Xu, Yao Nie and Xiaoqing Mu

ACS Catal., 2023, 13, 158
DOI: 10.1021/acscatal.2c04735

Structure‐Guided Evolution of Cyclohexanone Monooxygenase Toward Bulky Omeprazole Sulfide: Substrate Migration and Stereoselectivity Inversion

Shiyu Wei, Guochao Xu, Jieyu Zhou and Ye Ni

ChemPhysChem, 2024, 25
DOI: 10.1002/cphc.202400008

Structure-guided steric hindrance engineering of Bacillus badius phenylalanine dehydrogenase for efficient l-homophenylalanine synthesis

Tao Wu, Xiaoqing Mu, Yuyan Xue, Yan Xu and Yao Nie

Biotechnol Biofuels, 2021, 14
DOI: 10.1186/s13068-021-02055-0

Rational design of imine reductase for asymmetric synthesis of α-methyl-benzylaminonitrogen heterocycles

Qinzhe Lin, Xuanjin Lv, Xianming Zeng, Mengning Zhong, Qiyun Wu, Huilin Ren, Shenpeng Xu, Wentian Chen, Wenting Du and Jun Li

Molecular Catalysis, 2024, 552, 113678
DOI: 10.1016/j.mcat.2023.113678

Engineering a Protease K for Efficient Degradation of Wool Scale Layer Using a Substrate Pocket Modification

Lei Zhao, Xiangyang Ma, Yunan Ding, Kaixin Zheng, Kefen Wang, Fuping Lu and Yihan Liu

Fermentation, 2025, 11, 51
DOI: 10.3390/fermentation11020051

Efficient and easible biocatalysts: Strategies for enzyme improvement. A review

Samah Hashim Albayati, Nima Ghahremani Nezhad, Anmar Ghanim Taki and Raja Noor Zaliha Raja Abd Rahman

International Journal of Biological Macromolecules, 2024, 276, 133978
DOI: 10.1016/j.ijbiomac.2024.133978

Structure-Guided Steric Hindrance Engineering of Devosia Strain A6–243 Quinone-Dependent Dehydrogenase to Enhance Its Catalytic Efficiency

Jiafeng Niu, Bin Ma, Juan Shen, Huibing Chi, Huimin Zhou, Zhaoxin Lu, Fengxia Lu and Ping Zhu

J. Agric. Food Chem., 2024, 72, 549
DOI: 10.1021/acs.jafc.3c07179

Mirror substrates specificity of a 2, 3-dihydroxypropanesulfonate degrading enzyme in sulfate-reducing bacteria

Xiaoyi Ma, Huanyu Wang, Le Liu, Hongyue Dang and Kai Tang

International Journal of Biological Macromolecules, 2025, 306, 141806
DOI: 10.1016/j.ijbiomac.2025.141806

Stairway to Stereoisomers: Engineering Short‐ and Medium‐Chain Ketoreductases To Produce Chiral Alcohols

Anirudh P. Shanbhag

ChemBioChem, 2023, 24
DOI: 10.1002/cbic.202200687

Alcohol dehydrogenases catalyzed asymmetric reductions of “difficult-to-reduce” ketones and the involved mechanism of stereoselective recognition and regulation

Xin Su, Jie Gu, Yan Xu and Yao Nie

Critical Reviews in Biotechnology, 2026, 1
DOI: 10.1080/07388551.2026.2638569

Enzymatic synthesis of chiral alcohols using ketoreductases

Quan Yuan, Li Ma, Weixi Kong, Jianqiao Liu, Shuling Zhang, Jiajing Yan, Jing Bai, Ying He, Liya Zhou, Yunting Liu and Yanjun Jiang

Catalysis Reviews, 2025, 67, 283
DOI: 10.1080/01614940.2024.2313603

Enhancing the Activity of an Alcohol Dehydrogenase by Using “Aromatic Residue Scanning” at Potential Plasticity Sites

Wen‐Jie Ye, Jing‐Wen Xie, Yan Liu, Yi‐Lin Wang, Yu‐Xin Zhang, Xiao‐Ying Yang, Lin Yang, Hua‐Lei Wang and Dong‐Zhi Wei

Chemistry A European J, 2023, 29
DOI: 10.1002/chem.202203530

Asymmetric synthesis of intermediate for (1R,2S)-ethyl 1-amino-2-vinylcyclopropanecarboxylate by desymmetrization using engineered esterase from Bacillus subtilis

Hiroshi Kawabata, Ryoma Miyake, Kuniko Asada, Yasumasa Dekishima, Mitsuko Miyaike and Ryohei Kato

Journal of Bioscience and Bioengineering, 2021, 131, 599
DOI: 10.1016/j.jbiosc.2021.02.004

Efficient synthesis of bepotastine and cloperastine intermediates using engineered alcohol dehydrogenase with a hydrophobic pocket

Kai Wu, Jinrong Yan, Xiaojing Wang, Xinai Yin, Guixiang Shi, Lei Yang, Fangling Li, Junhai Huang and Lei Shao

Appl Microbiol Biotechnol, 2021, 105, 5873
DOI: 10.1007/s00253-021-11413-9

Rational design of enzyme activity and enantioselectivity

Zhongdi Song, Qunfeng Zhang, Wenhui Wu, Zhongji Pu and Haoran Yu

Front. Bioeng. Biotechnol., 2023, 11
DOI: 10.3389/fbioe.2023.1129149

Shield Machine-like Substrate Walking Strategy-Based Pocket Engineering of F-Amine Dehydrogenase for Accessing Structurally Diverse Fused-Ring and Linked-Ring Aryl Ketones

Tao Wu, Yan Xu, Yao Nie and Xiaoqing Mu

ACS Catal., 2024, 14, 2685
DOI: 10.1021/acscatal.4c00068

Enhancing Stability and Catalytic Activity of d-Allulose 3-Epimerase through Multistrategy Computational Design and Cross-Regional Advantageous Mutations

Zhiqi Wang, Tingting Feng, Longyan Zhao, Ning Li and Jidong Liu

J. Agric. Food Chem., 2025, 73, 635
DOI: 10.1021/acs.jafc.4c07342

Rational redesign of the loop dynamics of carbonyl reductase LfSDR1 to improve the stereoselectivity for asymmetric synthesis of bulky chiral alcohols

Xiaoping Yue, Yitong Li, Mankun Wei, Yu Duan, Lin Yang and Fen-Er Chen

International Journal of Biological Macromolecules, 2024, 274, 133345
DOI: 10.1016/j.ijbiomac.2024.133345

Redesign of (R)-Omega-Transaminase and Its Application for Synthesizing Amino Acids with Bulky Side Chain

Dong-Xu Jia, Chen Peng, Jun-Liang Li, Fan Wang, Zhi-Qiang Liu and Yu-Guo Zheng

Appl Biochem Biotechnol, 2021, 193, 3624
DOI: 10.1007/s12010-021-03616-7

Stereodivergent evolution of KpADH for the asymmetric reduction of diaryl ketones with para-substituents

Jiacheng Zhang, Jieyu Zhou, Guochao Xu and Ye Ni

Molecular Catalysis, 2022, 524, 112315
DOI: 10.1016/j.mcat.2022.112315

Engineering a Medium‐chain Alcohol Dehydrogenase for Efficient Synthesis of (S)‐N−Boc‐3−pyrrolidinol by Adjusting the Conformational Dynamics of Loops

Wenjie Ye, Yuxin Zhang, Yilin Wang, Jingwen Xie, Yan Liu, Lin Yang, Hualei Wang and Dongzhi Wei

ChemCatChem, 2023, 15
DOI: 10.1002/cctc.202201175

Effect and mechanism of mercaptobenzoheterocyclic derivatives as brighteners in acid sulfate tin electroplating

Yuxuan Huang, Zhihua Tao, Zewei Lin, Songlin Zhang, Hongwei Chen, Tianpeng Liang, Libin Gao and Jihua Zhang

Electrochimica Acta, 2025, 531, 146459
DOI: 10.1016/j.electacta.2025.146459