Issue 6, 2021

Interrogating the transcriptome with metabolically incorporated ribonucleosides

Abstract

RNA is a central player in biological processes, but there remain major gaps in our understanding of transcriptomic processes and the underlying biochemical mechanisms regulating RNA in cells. A powerful strategy to facilitate molecular analysis of cellular RNA is the metabolic incorporation of chemical probes. In this review, we discuss current approaches for RNA metabolic labeling with modified ribonucleosides and their integration with Next-Generation Sequencing, mass spectrometry-based proteomics, and fluorescence microscopy in order to interrogate RNA behavior in its native context.

Graphical abstract: Interrogating the transcriptome with metabolically incorporated ribonucleosides

Article information

Article type
Review Article
Submitted
20 Ago 2021
Accepted
07 Okt 2021
First published
07 Okt 2021

Mol. Omics, 2021,17, 833-841

Author version available

Interrogating the transcriptome with metabolically incorporated ribonucleosides

R. E. Kleiner, Mol. Omics, 2021, 17, 833 DOI: 10.1039/D1MO00334H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements