Issue 22, 2018

Ultrasensitive electrochemical detection of miRNA based on DNA strand displacement polymerization and Ca2+-dependent DNAzyme cleavage

Abstract

miRNAs are novel diagnostic and prognostic biomarkers for a diversity of diseases like cancers. In this study, an ultrasensitive electrochemical biosensor for miRNA evaluation is fabricated. A methylene blue (MB) labeled DNA probe is firstly modified on a gold electrode. By employing target induced strand displacement amplification and subsequent DNAzyme cleavage cycles, a large number of MB molecules are released. The decreased oxidation peak current could be used to reveal the miRNA concentration. Based on the two efficient signal amplifications, this method shows ultrahigh sensitivity. Its feasibility for the analysis of miRNA in cell lysates is also demonstrated. Therefore, the method shows attractive potential for promising applications in early diagnosis of certain diseases.

Graphical abstract: Ultrasensitive electrochemical detection of miRNA based on DNA strand displacement polymerization and Ca2+-dependent DNAzyme cleavage

Supplementary files

Article information

Article type
Communication
Submitted
12 Ago 2018
Accepted
19 Sep 2018
First published
25 Sep 2018

Analyst, 2018,143, 5352-5357

Ultrasensitive electrochemical detection of miRNA based on DNA strand displacement polymerization and Ca2+-dependent DNAzyme cleavage

D. Yang, W. Cheng, X. Chen, Y. Tang and P. Miao, Analyst, 2018, 143, 5352 DOI: 10.1039/C8AN01555D

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