Issue 22, 2023

Hairpin DNA-based electrochemical amplification strategy for miRNA sensing by using single gold nanoelectrodes

Abstract

A new sensor has been developed to detect miRNA-15 using nanoelectrodes and a hairpin DNA-based electrochemical amplification technique. By utilizing a complex DNA cylinder connected with hairpin DNA1, the sensor is able to absorb more methylene blue (MB) than simple double-stranded DNA. Another hairpin DNA2 is modified on an Au nanoelectrode surface and, when miRNA-15 is introduced, it triggers a chain reaction. This reaction unlocks two hairpins alternatively to polymerize into a complex structure that attaches more MB. The miRNA-15 is then replaced by DNA1 due to strand displacement reactions and continues to react with the next DNA2 to achieve circular amplification. The electrochemical signal from MB oxidation has a linear relationship with the miRNA-15 concentrations, making it possible to detect miRNA-15. Moreover, this method can be readily adapted for the detection of various other miRNA species. The newly devised nanosensor holds promising applications for the in vivo detection of miRNA-15 within biological systems, which is achieved by leveraging the advantageous characteristics of nanoelectrodes, including their low resistance–capacitance time constant, rapid mass transfer kinetics, and small diameter.

Graphical abstract: Hairpin DNA-based electrochemical amplification strategy for miRNA sensing by using single gold nanoelectrodes

Supplementary files

Article information

Article type
Paper
Submitted
19 Sep 2023
Accepted
10 Okt 2023
First published
12 Okt 2023

Analyst, 2023,148, 5636-5641

Hairpin DNA-based electrochemical amplification strategy for miRNA sensing by using single gold nanoelectrodes

H. Wang, B. Yang, H. Tang, S. Ding and G. Liu, Analyst, 2023, 148, 5636 DOI: 10.1039/D3AN01551C

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