Issue 89, 2022

Residue-specific N-terminal glycine to aldehyde transformation renders analytically pure single-site labeled proteins

Abstract

Here, we present N-Gly-specific glyoxamide generation in native proteins, isolated or in a complex mixture. The resulting aldehyde enables parallel installation of probes and a purification platform to render analytically pure single-site tagged proteins. It renders N-Gly engineered insulin without perturbing its structure, receptor binding, and downstream signaling pathway.

Graphical abstract: Residue-specific N-terminal glycine to aldehyde transformation renders analytically pure single-site labeled proteins

Supplementary files

Article information

Article type
Communication
Submitted
27 Jul 2022
Accepted
13 Okt 2022
First published
17 Okt 2022

Chem. Commun., 2022,58, 12451-12454

Residue-specific N-terminal glycine to aldehyde transformation renders analytically pure single-site labeled proteins

T. Sahu, M. Kumar, S. T. K., M. Joshi, R. K. Mishra and V. Rai, Chem. Commun., 2022, 58, 12451 DOI: 10.1039/D2CC04196K

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