Issue 46, 2022

Covalent organic framework linked with amination luminol derivative as enhanced ECL luminophore for ultrasensitive analysis of cytochrome c

Abstract

Cytochrome c (cyt c) plays a critical role in mitochondrial respiratory chain, whose absence is detrimental to electron transport and reduce adenosine triphosphate. For ultrasensitive detection of cyt c, sheet-like covalent organic frameworks (COFs) were prepared by orderly accumulation of 1,3,5-benzenetricarboxaldehyde (BTA) and p-phenylenediamine (PDA), and further grafted with N-(4-aminobutyl)-N-ethylisoluminol (ABEI) – an electrochemiluminescence (ECL) emitter. Specifically, the morphology and structure of the COFs-ABEI were mainly characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) analysis, and X-ray photoelectron spectroscopy (XPS). In parallel, the optical properties of the emitter were certified by UV-vis absorbance spectroscopy, Fourier infrared spectroscopy (FTIR), fluorescence (FL), and ECL measurements, showing 2.25-time enhanced ECL efficiency over pure ABEI, coupled by illustrating the interfacial electron transport mechanism. On the above foundation, a label-free “signal off” ECL biosensor was constructed by virtue of the specific immune recognition between the aptamer of the target cyt c with its capture DNA (cDNA) anchored on the biosensing platform, exhibiting a wider linear range of 1.00 fg mL−1–0.10 ng mL−1 (R2 = 0.998) and a lower limit of detection (LOD) down to 0.73 fg mL−1. This work offers some constructive guidelines for sensitive bioassays of disease-related biomarkers in the clinical field.

Graphical abstract: Covalent organic framework linked with amination luminol derivative as enhanced ECL luminophore for ultrasensitive analysis of cytochrome c

Supplementary files

Article information

Article type
Paper
Submitted
28 Jul 2022
Accepted
21 Okt 2022
First published
23 Nov 2022

Anal. Methods, 2022,14, 4767-4774

Covalent organic framework linked with amination luminol derivative as enhanced ECL luminophore for ultrasensitive analysis of cytochrome c

L. Shen, Y. Wang, H. Shan, J. Chen, A. Wang, W. Liu, P. Yuan and J. Feng, Anal. Methods, 2022, 14, 4767 DOI: 10.1039/D2AY01208A

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