Issue 23, 2022

A bioluminescent probe for NQO1 overexpressing cancer cell imaging in vitro and in vivo

Abstract

NAD(P)H quinone oxidoreductase1 (NQO1) is a flavoenzyme that regulates the redox potential level in cells. Overexpression of NQO1 has been proven to be relevant to some malignant tumors. Herein a bioluminescent probe NQO1-Luc equipped with an NQO1-targeting group, trimethyl-locked quinone propionic acid (QPA), was constructed. The reduction of NQO1-Luc could be triggered by NQO1, resulting in the release of free D-luciferin, and concomitantly a bright bioluminescence emission. NQO1-Luc exhibits high selectivity and sensitivity toward NQO1 activity and the capability of distinguishing NQO1-overexpressing cells in vitro and in vivo, which offers a promising tool for investigations of NQO1 related biological processes including tumors in living organisms.

Graphical abstract: A bioluminescent probe for NQO1 overexpressing cancer cell imaging in vitro and in vivo

Supplementary files

Article information

Article type
Communication
Submitted
31 Ago 2022
Accepted
26 Okt 2022
First published
28 Okt 2022

Analyst, 2022,147, 5264-5268

A bioluminescent probe for NQO1 overexpressing cancer cell imaging in vitro and in vivo

Y. Luo, W. Wang, Y. Zeng, S. Wang, X. Guo, R. Hu and G. Yang, Analyst, 2022, 147, 5264 DOI: 10.1039/D2AN01435A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements