Issue 24, 2020

In cell Gd3+-based site-directed spin labeling and EPR spectroscopy of eGFP

Abstract

Label-based functional studies of biomolecules in their native environment require labeling reactions inside living cells. In cell spin labeling using alkyne–azide click chemistry with a Gd3+-DOTAM-azide complex is shown to provide high spin label stability and narrow EPR lines for EPR spectroscopic detection of a spin labeled protein in living cells at ambient temperatures.

Graphical abstract: In cell Gd3+-based site-directed spin labeling and EPR spectroscopy of eGFP

Supplementary files

Article information

Article type
Communication
Submitted
09 Apr 2020
Accepted
04 Mei 2020
First published
01 Jun 2020
This article is Open Access
Creative Commons BY license

Phys. Chem. Chem. Phys., 2020,22, 13358-13362

In cell Gd3+-based site-directed spin labeling and EPR spectroscopy of eGFP

S. Kucher, S. Korneev, J. P. Klare, D. Klose and H. Steinhoff, Phys. Chem. Chem. Phys., 2020, 22, 13358 DOI: 10.1039/D0CP01930E

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