Issue 55, 2020

Enzymatic modular synthesis and microarray assay of poly-N-acetyllactosamine derivatives

Abstract

A facile enzymatic modular assembly strategy for the preparative-scale synthesis of poly-N-acetyllactosamine (poly-LacNAc) glycans with varied lengths and designed sialylation and/or fucosylation patterns is described. These glycans were printed as a microarray to investigate their interactions with a panel of glycan binding proteins (GBPs). Binding affinities revealed that the avidity of GBPs could be largely affected by the length and the patterns of sialylation and fucosylation.

Graphical abstract: Enzymatic modular synthesis and microarray assay of poly-N-acetyllactosamine derivatives

Supplementary files

Article information

Article type
Communication
Submitted
06 Mei 2020
Accepted
15 Jun 2020
First published
15 Jun 2020

Chem. Commun., 2020,56, 7549-7552

Enzymatic modular synthesis and microarray assay of poly-N-acetyllactosamine derivatives

C. Chen, S. Wang, M. R. Gadi, H. Zhu, F. Liu, C. Liu, L. Li, F. Wang, P. Ling and H. Cao, Chem. Commun., 2020, 56, 7549 DOI: 10.1039/D0CC03268A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements