Issue 1, 2019

RNA chaperone assisted intramolecular annealing reaction towards oligouridylated RNA detection in cancer cells

Abstract

Proximity induced intramolecular nucleotide strand displacement, which can be simply performed in a single tube or in a complex cellular environment, is one of the key mechanisms for the detection of biological targets, especially for significant genetic molecules. The host factor for RNA phage Qb replication (Hfq), with two distinct single stranded RNA binding sites, has excellent properties as an affinity ligand in a proximity induced reaction. In this research, a versatile RNA chaperone-Hfq assisted RNA annealing strategy for the sensitive detection of the intermediate product, oligouridylated RNA, in a genetic regulation process was developed. Benefiting from the high binding affinity of Hfq for the probe and the target, the sensitive determination of oligouridylated RNA in cell lysis and human cervical cancer (HeLa) cells was successfully achieved. This study has also revealed that the Hfq assisted RNA annealing strategy can be further extended and applied in specific microRNA analysis, and RNA related tumorigenicity and disease diagnosis.

Graphical abstract: RNA chaperone assisted intramolecular annealing reaction towards oligouridylated RNA detection in cancer cells

Supplementary files

Article information

Article type
Paper
Submitted
28 Ago 2018
Accepted
19 Okt 2018
First published
22 Okt 2018

Analyst, 2019,144, 186-190

RNA chaperone assisted intramolecular annealing reaction towards oligouridylated RNA detection in cancer cells

K. Zhang, X. Yang, T. Zhang, X. Li, H. Chen and J. Xu, Analyst, 2019, 144, 186 DOI: 10.1039/C8AN01662C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements