Issue 11, 2018

Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

Abstract

We have created modified protein variants by introducing a non-canonical amino acid p-azido-L-phenylalanine (azF) into defined positions for photochemically-induced covalent attachment to graphene. Attachment of GFP, TEM and cyt b562 proteins was verified through a combination of atomic force and scanning tunnelling microscopy, resistance measurements, Raman data and fluorescence measurements. This method can in principle be extended to any protein which can be engineered in this way without adversely affecting its structural stability.

Graphical abstract: Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

Supplementary files

Article information

Article type
Paper
Submitted
10 Okt 2017
Accepted
11 Jan 2018
First published
05 Feb 2018
This article is Open Access
Creative Commons BY license

RSC Adv., 2018,8, 5768-5775

Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

A. J. Zaki, Andrew M. Hartley, S. C. Reddington, S. K. Thomas, P. Watson, A. Hayes, A. V. Moskalenko, M. F. Craciun, J. E. Macdonald, D. D. Jones and M. Elliott, RSC Adv., 2018, 8, 5768 DOI: 10.1039/C7RA11166E

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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