Issue 3, 2017

Influence of protein adsorption on the cellular uptake of AuNPs conjugated with chiral oligomers

Abstract

Upon nanoparticles's (NPs) encounter with biological fluids, proteins shall be inevitably adsorbed on their surface to form a protein corona. Although its importance is widely accepted, information on the influence of the surface chirality of NPs on the protein corona and thereby cellular uptake is still missing. Herein, poly(acryloyl-L(D)-valine (L(D)-PAV) and poly(acryloyl-L(D)-valine)-b-poly(2-hydroxyethyl methylacrylate) (L(D)-PAV-b-PHEMA) chiral molecules were conjugated on a gold NP (AuNP) surface. Their interaction with serum proteins and A549 and HepG2 cancer cells in a medium containing concentrated serum was studied. The proteins in the serum-rich medium largely adsorbed onto PAV–AuNP NPs regardless of their optical activity, and shielded the chiral-selectivity in terms of cellular uptake. The introduction of a hydrophilic PHEMA block underneath the PAV block could significantly reduce the adsorption of serum proteins, and maintain the chiral-selective cellular uptake of PAV-b-PHEMA–AuNPs, indicating efficient performance by combining non-specific repelling and chiral-targeting effects.

Graphical abstract: Influence of protein adsorption on the cellular uptake of AuNPs conjugated with chiral oligomers

Supplementary files

Article information

Article type
Research Article
Submitted
07 Ago 2016
Accepted
05 Sep 2016
First published
15 Sep 2016

Mater. Chem. Front., 2017,1, 542-549

Influence of protein adsorption on the cellular uptake of AuNPs conjugated with chiral oligomers

J. Deng, H. Zheng and C. Gao, Mater. Chem. Front., 2017, 1, 542 DOI: 10.1039/C6QM00163G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements