Eirini Antonatou, Yentl Verleysen and Annemieke Madder
Org. Biomol. Chem., 2017,15, 8140-8144
DOI:
10.1039/C7OB02245J,
Paper
We here describe a furan oxidation based site-specific chemical ligation approach using unprotected peptide segments. This approach involves two steps: after photooxidation of a furan-containing peptide, ligation is achieved by reaction of the unmasked keto–enal with C- or N-terminal α-nucleophilic moieties of the second peptide such as hydrazine or hydrazide to form a pyridazinium or pyrrolidinone linkage respectively.
