Issue 6, 2016

Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3

Abstract

A site-specific dual-color labeled ubiquitin for sensing deubiquitinase's activity was prepared by consecutively using chemoselective native chemical ligation reactions in a facile and efficient way. The prepared sensor was applied to establish a sensitive FRET-based assay for UCH-L3.

Graphical abstract: Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3

Supplementary files

Article information

Article type
Communication
Submitted
03 Mac 2016
Accepted
13 Apr 2016
First published
13 Apr 2016

Mol. BioSyst., 2016,12, 1764-1767

Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3

L. Li, S. Zhang, Y. Li and Y. Chen, Mol. BioSyst., 2016, 12, 1764 DOI: 10.1039/C6MB00165C

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