Issue 58, 2016
From the journal:

Chemical Communications

Development of a disaggregation-induced emission probe for the detection of RecA inteins from Mycobacterium tuberculosis†

Xin Li,a   Hao Jiang,b   Zhaofeng Luo,b   Young-Tae Changc  and  Liyun Zhang*a  

* Corresponding authors

a Institute of Technical Biology and Agriculture Engineering, Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, P. R. China
E-mail: zly0605@ustc.edu.cn
Fax: +86-551-63603125
Tel: +86-551-63607335

b Hefei National Laboratory for Physical Science at the Microscale, Core Facility Center for Life Sciences, School of Life Sciences, University of Science and Technology of China, Hefei 230026, P. R. China

c Department of Chemistry, National University of Singapore, 3 Science Drive 2, Singapore

Abstract

A fluorescent sensor – InR – with disaggregation-induced emission (DIE) characteristics has been developed for the label free detection of RecA inteins from Mycobacterium tuberculosis. Mechanistic studies demonstrate that InR binds at a hydrophobic pocket of RecA inteins, which is constituted by Gly435, Glu434, Arg405 and Arg 7, resulting in the disaggregation and recovery of its fluorescence.

Graphical abstract: Development of a disaggregation-induced emission probe for the detection of RecA inteins from Mycobacterium tuberculosis

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Article information

DOI
https://doi.org/10.1039/C6CC00439C
Article type
Communication
Submitted
16 Jan 2016
Accepted
19 Feb 2016
First published
19 Feb 2016

Chem. Commun., 2016,52, 9086-9088

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Development of a disaggregation-induced emission probe for the detection of RecA inteins from Mycobacterium tuberculosis

X. Li, H. Jiang, Z. Luo, Y. Chang and L. Zhang, Chem. Commun., 2016, 52, 9086 DOI: 10.1039/C6CC00439C

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