Issue 1, 2015

An internal standardisation strategy for quantitative immunoassay tissue imaging using laser ablation inductively coupled plasma mass spectrometry

Abstract

Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) for imaging of biological materials, such as tissue sections, has gained growing attention in the last few years, and even more since the coupling with isotopically enriched and labelled antibodies for quantitative immunoassays became available. In this work, an internal standardisation strategy for the quantitative imaging analysis of biological samples by LA-ICP-MS using labelling with an iridium compound is proposed. The utilisation of an iridium intercalator offers the advantage that the internal standard does not only correct for drift and matrix effects but also correct for differences in the ablated mass at the same time. For this, the applicability of the internal standard was investigated for formalin fixed paraffin embedded tissues (FFPEs) and for cryosections. Background contamination and thickness correlation were studied. As a proof of concept, the quantification of an isotope labelled mouse anti-E-Cadherin antibody was performed on a FFPE kidney section of a mouse at different spatial resolutions.

Graphical abstract: An internal standardisation strategy for quantitative immunoassay tissue imaging using laser ablation inductively coupled plasma mass spectrometry

Supplementary files

Article information

Article type
Paper
Submitted
29 Ago 2014
Accepted
29 Sep 2014
First published
29 Sep 2014

J. Anal. At. Spectrom., 2015,30, 254-259

An internal standardisation strategy for quantitative immunoassay tissue imaging using laser ablation inductively coupled plasma mass spectrometry

D. A. Frick, C. Giesen, T. Hemmerle, B. Bodenmiller and D. Günther, J. Anal. At. Spectrom., 2015, 30, 254 DOI: 10.1039/C4JA00293H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements