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Abstract | Cited by

Mutants of toluene o-xylene monooxygenase are demonstrated to oxidize ethylene to ethylene oxide in vivo at yields of >99%. The best mutant increases ethylene oxidation activity by >5500-fold relative to the native enzyme. This is the first report of a recombinant enzyme capable of carrying out this industrially significant chemical conversion.

Graphical abstract: Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenase

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