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We show that direct coupling of a dye-labelled DNA (acceptor) to a quantum dot (QD) donor significantly reduces the donor–acceptor distance and improves the FRET efficiency: a highly efficient FRET (∼88%) at a low acceptor-to-donor ratio of 2 has been achieved at the single-molecule level.

Graphical abstract: Fluorescence resonance energy transfer between a quantum dot donor and a dye acceptor attached to DNA

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