Our study shows efficient tyrosine labeling using 1-methyl-4-arylurazole (MAUra) with laccase under mild conditions. This method achieves a high efficiency (kcat/Km = 7.88 × 104 M−1 s−1), selectively targeting exposed tyrosine sites on proteins.
We developed a system to separate and identify racemised and isomerised aspartic acid residues in amyloid β by labeling with an original chiral resolution labeling reagent, D-FDLDA.
We synthesized isoluminol derivatives with a terminal carboxyl group. The resulting NHS ester is a stable, efficient chemiluminescent reagent with high protein labelling efficacy.
We developed a method for the separation and highly sensitive detection of structural isomers and enantiomers of aminobutyric acid using a LC-MS with a C18 column by labeling with an original chiral resolution labeling reagent, L-FDVDA.
This highlight provides an account of the discovery and advances in the tyrosine-click (Y-click) reaction and its extensive application in the mapping of proteins, imaging of living cells, and in the fields of analytical and medicinal chemistry.