Issue 7, 2020

A novel NIR fluorescent probe for fast detection and imaging of methionine sulfoxide reductase A in lysosome of living cells

Abstract

Methionine sulfoxide reductases (Msrs) play special roles in protecting proteins from oxidative damage caused by reactive species and repairing MetSO residues in proteins/peptides. However, current probes for Msrs fail to target lysosomes and exhibit a relatively slow response and short emission wavelengths (less than 580 nm). Herein, we reported a novel lysosome-targeted NIR fluorescent probe RBSO for fast detection and imaging Msr A by extending the linear π-conjugated system of the probe via the SNArH reaction. As expected, RBSO exhibited high selectivity and high sensitivity for the rapid detection of Msr A in real-time via a specific Msr A-triggered methylsulfinyl reduction reaction. Importantly, RBSO was cell-membrane permeable and capable of specifically distributing in lysosomes, and thus detects Msr A in real-time there, providing a potential tool for monitoring Msr A activity in lysosomes of living cells.

Graphical abstract: A novel NIR fluorescent probe for fast detection and imaging of methionine sulfoxide reductase A in lysosome of living cells

Supplementary files

Article information

Article type
Paper
Submitted
08 jul 2020
Accepted
28 aug 2020
First published
02 sep 2020
This article is Open Access
Creative Commons BY-NC license

Mater. Adv., 2020,1, 2401-2406

A novel NIR fluorescent probe for fast detection and imaging of methionine sulfoxide reductase A in lysosome of living cells

J. Gong, C. Liu, S. Cai, S. He, L. Zhao and X. Zeng, Mater. Adv., 2020, 1, 2401 DOI: 10.1039/D0MA00489H

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