Issue 39, 2018

Tailor-made Janus lectin with dual avidity assembles glycoconjugate multilayers and crosslinks protocells

Abstract

We engineered the first chimeric, bispecific lectin, with two rationally oriented and distinct recognition surfaces. This lectin, coined Janus lectin in allusion to the two-faced roman god, is able to bind independently to both fucosylated and sialylated glycoconjugates. The multivalent presentation of binding sites on each face of the Janus lectin is very efficient, resulting in avidities in the low nanomolar range for both fucosylated and sialylated surfaces. Moreover, novel heterovalent, bifunctional glycoclusters were synthetized that match the topology of the Janus lectin. Based on these tools, we constructed organized and controlled supramolecular architectures by assembling Janus lectin and glycocompound layer-by-layer. Furthermore, the Janus lectin was employed as biomolecular linker to organize protocells made from giant unilamellar vesicles of different nature, to more complex prototissues. In summary, tailor-made Janus lectins open wide possibilities for creating biomimetic matrices or artificial tissues.

Graphical abstract: Tailor-made Janus lectin with dual avidity assembles glycoconjugate multilayers and crosslinks protocells

Supplementary files

Article information

Article type
Edge Article
Submitted
21 jun 2018
Accepted
12 aug 2018
First published
14 aug 2018
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2018,9, 7634-7641

Tailor-made Janus lectin with dual avidity assembles glycoconjugate multilayers and crosslinks protocells

J. P. Ribeiro, S. Villringer, D. Goyard, L. Coche-Guerente, M. Höferlin, O. Renaudet, W. Römer and A. Imberty, Chem. Sci., 2018, 9, 7634 DOI: 10.1039/C8SC02730G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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