Issue 1, 2018

Rapid, selective and stable HaloTag-LbADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides

Abstract

A strategy for biocatalyst immobilization in flow directly from the crude cell extract is described. The efficiency and the stability of the immobilized enzyme were demonstrated during the asymmetric reduction of a range of ketones. The cascade two-step chemo-enzymatic preparation of chiral epoxides was possible through the initial ketone bioreduction to an intermediate halohydrin followed by its intramolecular cyclization.

Graphical abstract: Rapid, selective and stable HaloTag-LbADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides

Supplementary files

Article information

Article type
Communication
Submitted
16 okt 2017
Accepted
07 dec 2017
First published
17 jan 2018
This article is Open Access
Creative Commons BY license

React. Chem. Eng., 2018,3, 8-12

Rapid, selective and stable HaloTag-LbADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides

J. Döbber, M. Pohl, S. V. Ley and B. Musio, React. Chem. Eng., 2018, 3, 8 DOI: 10.1039/C7RE00173H

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