Volume 207, 2018

Probing the excited state dynamics of Venus: origin of dual-emission in fluorescent proteins

Abstract

Fluorescent proteins exhibit interesting excited state photochemistry, leading to bright fluorescence emission that renders their versatile biological role and wide use as biomarkers. A molecular-level mechanism of the excited state dynamics is desirable to pinpoint the origin of the bright fluorescence of these proteins. Here we present studies on a yellow fluorescent protein variant, Venus, and investigate the photophysics behind the dual fluorescence emission upon UV excitation. Based on our studies, we propose that the unique nature of the potential energy surface is responsible for the observation of minor fluorescence in Venus which is not seen in wild type GFP.

Graphical abstract: Probing the excited state dynamics of Venus: origin of dual-emission in fluorescent proteins

Associated articles

Article information

Article type
Paper
Submitted
04 okt 2017
Accepted
06 nov 2017
First published
06 nov 2017

Faraday Discuss., 2018,207, 39-54

Probing the excited state dynamics of Venus: origin of dual-emission in fluorescent proteins

S. Dhamija, B. Thakur, P. Guptasarma and A. K. De, Faraday Discuss., 2018, 207, 39 DOI: 10.1039/C7FD00187H

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