Issue 71, 2018

ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite

Abstract

In this work, we set out to develop an endoplasmic reticulum (ER) directed ESIPT-based ONOO ratiometric fluorescent probe (ABAH-LW). ABAH-LW was synthesized in four steps and found to have a high sensitivity and selectivity towards the detection of ONOO. ABAH-LW was able to detect low concentrations of ONOO (limit of detection = 21.4 nM) within seconds producing a ratiometric change in fluorescence intensity. ABAH-LW further demonstrated the ability to ratiometrically image endogenous and exogenous ONOO in HeLa cells. Moreover, co-localization experiments were carried out using commercially available ER-Tracker Red, Lyso-Tracker Red and Mito-Tracker-Red, which were co-stained with ABAH-LW in HeLa cells. For ER-Tracker Red, Pearson's correlation co-efficient of 0.93 was determined and 3D surface plot analysis illustrated a large overlap between ABAH-LW and ER-Tracker Red using both red and blue channels. In addition some co-localisation with Mito-Tracker Red and ABAH-LW was observed (0.73).

Graphical abstract: ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite

Associated articles

Supplementary files

Article information

Article type
Communication
Submitted
20 jun 2018
Accepted
03 aug 2018
First published
07 aug 2018
This article is Open Access
Creative Commons BY license

Chem. Commun., 2018,54, 9953-9956

ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite

L. Wu, Y. Wang, M. Weber, L. Liu, A. C. Sedgwick, S. D. Bull, C. Huang and T. D. James, Chem. Commun., 2018, 54, 9953 DOI: 10.1039/C8CC04919J

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