Issue 86, 2015

Time-resolved confocal microscopy using lanthanide centred near-IR emission

Abstract

A method to uniquely identify signals originating from probes with different emission decay times in luminescence imaging has been developed. By using scanning confocal microscopy in combination with time-correlated single photon counting (TCSPC), Photon Arrival Time Imaging (PArTI) has been realised through off-line plotting of images using the photon arrival times. PArTI is the time-equivalent to spectrally resolved imaging, replacing the energy axis with a photon arrival time axis. Here, lanthanide probes were used to demonstrate the key advantages of the method. PArTI uses TCSPC data, involves no fitting, uses a single pulsed laser line for multicolour imaging, and can be used with a 100 millisecond dwell time per pixel.

Graphical abstract: Time-resolved confocal microscopy using lanthanide centred near-IR emission

Supplementary files

Article information

Article type
Communication
Submitted
09 jun 2015
Accepted
10 aug 2015
First published
17 aug 2015
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2015,5, 70282-70286

Time-resolved confocal microscopy using lanthanide centred near-IR emission

Z. Liao, M. Tropiano, S. Faulkner, T. Vosch and T. J. Sørensen, RSC Adv., 2015, 5, 70282 DOI: 10.1039/C5RA15759E

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements