Issue 21, 2015

Monoclonal antibody-based cross-reactive sandwich ELISA for the detection of Salmonella spp. in milk samples

Abstract

An immunogen consisting of Salmonella lipopolysaccharide and bovine serum albumin was prepared by periodate oxidation. Mice sera cross-reacted with strains of the genus Salmonella. Monoclonal antibodies (mAbs) against the Salmonella lipopolysaccharide core structure were obtained after cell fusion. Based on mAb 6E8, a cross-reactive sandwich enzyme-linked immunosorbent assay was developed. The detection limit of different strains in the genus Salmonella ranged from 1.56 × 106 to 1.25 × 107 colony-forming units ml−1. No cross-reactivity was observed with other bacterial strains tested, including Cronobacter sakazakii, Escherichia coli O6, E. coli strain O157 : H7, Campylobacter jejuni and Listeria monocytogenes. Samples of bovine milk spiked with 1 colony-forming unit ml−1Salmonella spp. were analysed following enrichment for 24 h.

Graphical abstract: Monoclonal antibody-based cross-reactive sandwich ELISA for the detection of Salmonella spp. in milk samples

Article information

Article type
Paper
Submitted
23 jul 2015
Accepted
13 sep 2015
First published
15 sep 2015

Anal. Methods, 2015,7, 9047-9053

Author version available

Monoclonal antibody-based cross-reactive sandwich ELISA for the detection of Salmonella spp. in milk samples

X. Wu, W. Wang, L. Liu, H. Kuang and C. Xu, Anal. Methods, 2015, 7, 9047 DOI: 10.1039/C5AY01923K

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