Issue 19, 2022

Glycan microarrays from construction to applications

Abstract

Through their specific interactions with proteins, cellular glycans play key roles in a wide range of physiological and pathological processes. One of the main goals of research in the areas of glycobiology and glycomedicine is to understand glycan–protein interactions at the molecular level. Over the past two decades, glycan microarrays have become powerful tools for the rapid evaluation of interactions between glycans and proteins. In this review, we briefly describe methods used for the preparation of glycan probes and the construction of glycan microarrays. Next, we highlight applications of glycan microarrays to rapid profiling of glycan-binding patterns of plant, animal and pathogenic lectins, as well as other proteins. Finally, we discuss other important uses of glycan microarrays, including the rapid analysis of substrate specificities of carbohydrate-active enzymes, the quantitative determination of glycan–protein interactions, discovering high-affinity or selective ligands for lectins, and identifying functional glycans within cells. We anticipate that this review will encourage researchers to employ glycan microarrays in diverse glycan-related studies.

Graphical abstract: Glycan microarrays from construction to applications

Article information

Article type
Review Article
Submitted
02 Jan 2022
First published
16 Leo 2022

Chem. Soc. Rev., 2022,51, 8276-8299

Glycan microarrays from construction to applications

Y. Kim, J. Y. Hyun and I. Shin, Chem. Soc. Rev., 2022, 51, 8276 DOI: 10.1039/D2CS00452F

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