Issue 21, 2022

Multi-wavelength fusion column fingerprint technology combined with chemometric analysis to evaluate the overall quality of the Gardenia jasminoides root

Abstract

Chromatographic fingerprinting provides effective technical means for quality evaluation of traditional Chinese medicine. In this work, a novel multi-wavelength fusion column fingerprint was obtained by intelligent selection of chromatographic peaks from different wavelengths, which displayed the maximum peak area information under the optimal wavelength at the same retention time. Here, the Gardenia jasminoides root was selected as a sample. The multi-wavelength fusion column fingerprint graph of the Gardenia jasminoides root was constructed from five wavelengths (203 nm, 210 nm, 238 nm, 250 nm and 330 nm). The peak capacity, peak resolution, the number of common peaks and similarity were used to evaluate the performance. The 19 batches of Gardenia jasminoides root were classified into three categories with clear distinction between origin categories based on the multi-wavelength fusion column fingerprint combined with chemometrics, including hierarchical cluster analysis and principal component analysis. Nine markers of variation that led to differences between batches were screened by orthogonal partial least squares discriminant analysis. This study demonstrated that the classification model based on the multi-wavelength fusion column fingerprint was better than that on a single-wavelength, and the fusion fingerprint was suitable for the identification and quality control of traditional Chinese medicine with more comprehensive chemical composition information and more accurate prediction ability.

Graphical abstract: Multi-wavelength fusion column fingerprint technology combined with chemometric analysis to evaluate the overall quality of the Gardenia jasminoides root

Supplementary files

Article information

Article type
Paper
Submitted
02 Ube 2022
Accepted
17 Mme 2022
First published
21 Mme 2022

Anal. Methods, 2022,14, 2051-2062

Multi-wavelength fusion column fingerprint technology combined with chemometric analysis to evaluate the overall quality of the Gardenia jasminoides root

H. Zhang, R. Bai, Y. Wu, J. Zeng, H. Jiang, X. Liu, H. Zhang and J. Yan, Anal. Methods, 2022, 14, 2051 DOI: 10.1039/D2AY00358A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements