Issue 23, 2022

Selective non-enzymatic uric acid sensing in the presence of dopamine: electropolymerized poly-pyrrole modified with a reduced graphene oxide/PEDOT:PSS composite

Abstract

A highly selective electrochemical sensor based on a molecularly imprinted polymer (MIP) to be developed for uric acid detection in the presence of dopamine as an interference molecule was demonstrated in this study. This non-enzymatic uric acid sensor was developed by electropolymerizing poly-pyrrole onto a composite of electrochemically reduced graphene oxide (ErGO) and poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) on a glassy carbon electrode (GCE) to give MIP/ErGO/PEDOT:PSS electrodes. The structural properties, surface morphology, and electrochemical interface of this fabricated uric acid sensor was then characterized using infrared spectroscopy, scanning electron microscopy, atomic force microscopy, and electrochemical impedance spectroscopy. This fabricated non-enzymatic electrochemical sensor (MIP/ErGO/PEDOT:PSS modified GCE) also showed excellent analytical performance at the optimum ratio of monomer/template concentration and optimized electropolymerization conditions, for example in the low concentration range of 0.1–100 μM with a detection limit of 0.05 μM towards uric acid detection in the presence of dopamine. Furthermore, this fabricated uric acid sensor also exhibited good reproducibility and stability for uric acid measurements in the presence of dopamine for 5 consecutive days. In addition, this sensor demonstrates highly selective detection of uric acid in the presence of several interfering species such as dopamine, urea, glucose, magnesium ions, and ascorbic acid. This fabricated uric acid sensor ultimately showed satisfactory uric acid measurement in samples of human urine and is expected to be used in early-stage disease diagnosis.

Graphical abstract: Selective non-enzymatic uric acid sensing in the presence of dopamine: electropolymerized poly-pyrrole modified with a reduced graphene oxide/PEDOT:PSS composite

Article information

Article type
Paper
Submitted
04 Leo 2022
Accepted
19 Mph 2022
First published
01 Pun 2022

Analyst, 2022,147, 5334-5346

Selective non-enzymatic uric acid sensing in the presence of dopamine: electropolymerized poly-pyrrole modified with a reduced graphene oxide/PEDOT:PSS composite

B. R. Putra, U. Nisa, R. Heryanto, M. Khalil, F. Khoerunnisa, A. Ridhova, Y. N. Thaha, F. Marken and W. T. Wahyuni, Analyst, 2022, 147, 5334 DOI: 10.1039/D2AN01463G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements