Issue 19, 2022

A tracer-type fluorescent probe for imaging adenosine triphosphate under the stresses of hydrogen sulfide and hydrogen peroxide in living cells

Abstract

Adenosine triphosphate (ATP) is a direct energy source in cells and the core of the biochemical system, and is closely related to various metabolic activities in living organisms. Therefore, designing a simple and rapid ATP detection method is significant to study its physiological function. Herein, a dual-channel fluorescent probe RhB-NA for the in situ imaging of ATP in living cells was designed and synthesized. When ATP bound to RhB-NA, the spirolactam in rhodamine B was induced to open, resulting in a new fluorescence response at 589 nm. Notably, in cell imaging, the treatment of HeLa cells with exogenous H2O2 and H2S, which have certain effects on the mitochondria, confirmed that RhB-NA could detect fluctuations in ATP levels after the mitochondrial state was affected. We believe that RhB-NA has far-reaching significance for studying certain physiological diseases caused by abnormal ATP levels.

Graphical abstract: A tracer-type fluorescent probe for imaging adenosine triphosphate under the stresses of hydrogen sulfide and hydrogen peroxide in living cells

Supplementary files

Article information

Article type
Paper
Submitted
02 Pha 2022
Accepted
15 Pha 2022
First published
16 Pha 2022

Analyst, 2022,147, 4222-4227

A tracer-type fluorescent probe for imaging adenosine triphosphate under the stresses of hydrogen sulfide and hydrogen peroxide in living cells

L. Yao, W. Zhang, C. Yin, Y. Zhang and F. Huo, Analyst, 2022, 147, 4222 DOI: 10.1039/D2AN01262F

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