Issue 23, 2023

Towards multimodal cellular imaging: optical and X-ray fluorescence

Abstract

Imaging techniques permit the study of the molecular interactions that underlie health and disease. Each imaging technique collects unique chemical information about the cellular environment. Multimodal imaging, using a single probe that can be detected by multiple imaging modalities, can maximise the information extracted from a single cellular sample by combining the results of different imaging techniques. Of particular interest in biological imaging is the combination of the specificity and sensitivity of optical fluorescence microscopy (OFM) with the quantitative and element-specific nature of X-ray fluorescence microscopy (XFM). Together, these techniques give a greater understanding of how native elements or therapeutics affect the cellular environment. This review focuses on recent studies where both techniques were used in conjunction to study cellular systems, demonstrating the breadth of biological models to which this combination of techniques can be applied and the potential for these techniques to unlock untapped knowledge of disease states.

Graphical abstract: Towards multimodal cellular imaging: optical and X-ray fluorescence

Article information

Article type
Review Article
Submitted
01 Upu 2023
First published
01 Pun 2023

Chem. Soc. Rev., 2023,52, 8295-8318

Towards multimodal cellular imaging: optical and X-ray fluorescence

M. E. Graziotto, C. J. Kidman, L. D. Adair, S. A. James, H. H. Harris and E. J. New, Chem. Soc. Rev., 2023, 52, 8295 DOI: 10.1039/D3CS00509G

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