Issue 23, 2022

A bioluminescent probe for NQO1 overexpressing cancer cell imaging in vitro and in vivo

Abstract

NAD(P)H quinone oxidoreductase1 (NQO1) is a flavoenzyme that regulates the redox potential level in cells. Overexpression of NQO1 has been proven to be relevant to some malignant tumors. Herein a bioluminescent probe NQO1-Luc equipped with an NQO1-targeting group, trimethyl-locked quinone propionic acid (QPA), was constructed. The reduction of NQO1-Luc could be triggered by NQO1, resulting in the release of free D-luciferin, and concomitantly a bright bioluminescence emission. NQO1-Luc exhibits high selectivity and sensitivity toward NQO1 activity and the capability of distinguishing NQO1-overexpressing cells in vitro and in vivo, which offers a promising tool for investigations of NQO1 related biological processes including tumors in living organisms.

Graphical abstract: A bioluminescent probe for NQO1 overexpressing cancer cell imaging in vitro and in vivo

Supplementary files

Article information

Article type
Communication
Submitted
31 Pha 2022
Accepted
26 Mph 2022
First published
28 Mph 2022

Analyst, 2022,147, 5264-5268

A bioluminescent probe for NQO1 overexpressing cancer cell imaging in vitro and in vivo

Y. Luo, W. Wang, Y. Zeng, S. Wang, X. Guo, R. Hu and G. Yang, Analyst, 2022, 147, 5264 DOI: 10.1039/D2AN01435A

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