Issue 22, 2022

A lysosome-targeting fluorescent probe to visualize endogenous and exogenous methylglyoxal in live cells and zebrafish

Abstract

The development of a lysosome-targeting fluorescent probe to visualize endogenous and exogenous methylglyoxal (MGO) in live cells has important implications for associated diseases. Herein, a lysosome-targeting fluorescent probe MGO-Naph-A was designed and synthesized to detect MGO with high selectivity. The probe contained naphthalimide as the fluorescent group, o-phenylenediamine as the MGO recognition group, and morpholine as the lysosome targeting group. This fluorescent probe could detect endogenous and exogenous MGO in living cells by precisely targeting and staining lysosomes. It could also detect MGO in living zebrafish. The results showed that the probe MGO-Naph-A has the potential to visualize MGO in lysosomes.

Graphical abstract: A lysosome-targeting fluorescent probe to visualize endogenous and exogenous methylglyoxal in live cells and zebrafish

Supplementary files

Article information

Article type
Communication
Submitted
22 Pha 2022
Accepted
08 Mph 2022
First published
14 Mph 2022

Analyst, 2022,147, 4949-4953

A lysosome-targeting fluorescent probe to visualize endogenous and exogenous methylglyoxal in live cells and zebrafish

Z. Luo, Z. Zhu, T. Zhang, H. Jiang, N. Huang, F. Liang, Z. Wang, Y. Li, X. He and S. Qian, Analyst, 2022, 147, 4949 DOI: 10.1039/D2AN01386J

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