Issue 20, 2022

Positive feedback drives a secondary nonlinear product burst during a biphasic DNA amplification reaction

Abstract

Isothermal DNA amplification reactions are used in a broad variety of applications, from diagnostic assays to DNA circuits, with greater speed and less complexity than established PCR technologies. We recently reported a unique, high gain, biphasic isothermal DNA amplification reaction, called the Ultrasensitive DNA Amplification Reaction (UDAR). Here we present a detailed analysis of the UDAR reaction pathways that initiates with a first phase followed by a nonlinear product burst, which is caused by an autocatalytic secondary reaction. The experimental reaction output was reproduced using an ordinary differential equation model based on detailed reaction mechanisms. This model provides insight on the relative importance of each reaction mechanism during both phases, which could aid in the design of product output during DNA amplification reactions.

Graphical abstract: Positive feedback drives a secondary nonlinear product burst during a biphasic DNA amplification reaction

Supplementary files

Article information

Article type
Paper
Submitted
30 Jan 2022
Accepted
15 Leo 2022
First published
20 Leo 2022

Analyst, 2022,147, 4450-4461

Author version available

Positive feedback drives a secondary nonlinear product burst during a biphasic DNA amplification reaction

B. Özay, S. D. Murphy, E. E. Stopps, T. Gedeon and S. E. McCalla, Analyst, 2022, 147, 4450 DOI: 10.1039/D2AN01067D

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