Issue 20, 2018

Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes

Abstract

As a mammalian toll-like receptor family member protein, TLR2 recognizes lipoproteins from bacteria and modulates the immune response by inducing the expression of various cytokines. We have developed fluorescence-labeled TLR2 ligands with either hydrophilic or hydrophobic fluorescence groups. The labeled ligands maintained the inflammatory IL-6 induction activity and enabled us to observe the internalization and colocalization of the TLR2 ligands using live-cell imaging. The time-lapse monitoring in the live-cell imaging of the fluorescence-labeled TLR2 ligand showed that TLR2/CD14 expression in the host cells enhanced the internalization of TLR2 ligand molecules.

Graphical abstract: Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes

Supplementary files

Article information

Article type
Paper
Submitted
30 Tsh 2017
Accepted
02 Mot 2018
First published
05 Mot 2018

Org. Biomol. Chem., 2018,16, 3824-3830

Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes

Y. Arai, K. Yokoyama, Y. Kawahara, Q. Feng, I. Ohta, A. Shimoyama, S. Inuki, K. Fukase, K. Kabayama and Y. Fujimoto, Org. Biomol. Chem., 2018, 16, 3824 DOI: 10.1039/C7OB03205F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements