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Given the importance of nanomaterials for medicine, health and biological sciences, a method for reliable intra-cellular tracking of nanocarbon materials at ultra-low concentrations was investigated. Namely, hyperspectral imaging of DNA-functionalized single-walled carbon nanotubes inside neural stem cells was demonstrated, over several mitosis cycles, also in a 3D z-stacking mode.

Graphical abstract: Micro-Raman spectroscopy as an enabling tool for long-term intracellular studies of nanomaterials at nanomolar concentration levels

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