Issue 7, 2013

Electrochemically assisted bacteria encapsulation in thin hybrid sol–gel films

Abstract

A novel method, based on the electrochemical manipulation of the sol–gel process, was developed to immobilize bacteria in thin hybrid sol–gel films. This enabled the safe immobilization of Escherichia coli on electrode surfaces. E. coli strains C600, MG1655 pUCD607 and MG1655 pZNTA-GFP were incorporated and physically encapsulated in a hybrid sol–gel matrix and the metabolic activity and membrane integrity of the bacteria were assessed as a function of the aging time in the absence of nutrients at +4 °C or −80 °C. LIVE/DEAD BacLight bacterial viability analysis detected by epi-fluorescence microscopy indicated the preservation of 95% of E. coli C600 membrane integrity in the sol–gel film. The presence of chitosan, trehalose and polyethylene glycol additives was shown to strongly improve the viability of E. coli cells in the electrodeposited matrix for 1 month after encapsulation. Finally, the bioluminescent activity of E. coli MG1655 pUCD607 was preserved by approximately 50% of the cells present in such composite films.

Graphical abstract: Electrochemically assisted bacteria encapsulation in thin hybrid sol–gel films

Article information

Article type
Paper
Submitted
16 Pun 2012
Accepted
06 Tsh 2012
First published
07 Tsh 2012

J. Mater. Chem. B, 2013,1, 1052-1059

Electrochemically assisted bacteria encapsulation in thin hybrid sol–gel films

W. Ghach, M. Etienne, P. Billard, F. P. A. Jorand and A. Walcarius, J. Mater. Chem. B, 2013, 1, 1052 DOI: 10.1039/C2TB00421F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements