Issue 85, 2023

Development of a whole-cell biosensor for β-lactamase inhibitor discovery

Abstract

The production of β-lactamases by bacterial pathogens endangers antimicrobial therapy, and new inhibitors for β-lactamases are urgently needed. We report the development of a luminescent-based biosensor that quantifies β-lactamase inhibition in a cellular context, based on the activation of transcriptional factor AmpR following the exposure of bacterial cells to β-lactams. This rapid method can account for factors like membrane permeability and can be employed to identify new β-lactamase inhibitors.

Graphical abstract: Development of a whole-cell biosensor for β-lactamase inhibitor discovery

Supplementary files

Article information

Article type
Communication
Submitted
25 Upu 2023
Accepted
02 Mph 2023
First published
02 Mph 2023

Chem. Commun., 2023,59, 12707-12710

Development of a whole-cell biosensor for β-lactamase inhibitor discovery

M. A. Jeffs, R. A. V. Gray, P. M. Sheth and C. T. Lohans, Chem. Commun., 2023, 59, 12707 DOI: 10.1039/D3CC03583B

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